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Inhibition of NLRP3 shifted the differentiation of naïve <t>CD4</t> + T cells toward a Treg phenotype in vitro. ( a ) Flow cytometry plots identifying Th17 and Treg populations in EAM versus MCC950-treated groups; ( b ) Bar graphs quantifying Th17 and Treg frequencies in EAM versus MCC950-treated groups; ( c ) Secreted IL-17 and IL-10 protein levels in culture supernatants; ( d ) RORγt and Foxp3 mRNA expression levels. Data are presented as mean ± SD, n = 3. NS indicates no statistical significance; ∗p < 0.05, ∗∗p < 0.01.
Naive Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Inhibition of NLRP3 shifted the differentiation of naïve <t>CD4</t> + T cells toward a Treg phenotype in vitro. ( a ) Flow cytometry plots identifying Th17 and Treg populations in EAM versus MCC950-treated groups; ( b ) Bar graphs quantifying Th17 and Treg frequencies in EAM versus MCC950-treated groups; ( c ) Secreted IL-17 and IL-10 protein levels in culture supernatants; ( d ) RORγt and Foxp3 mRNA expression levels. Data are presented as mean ± SD, n = 3. NS indicates no statistical significance; ∗p < 0.05, ∗∗p < 0.01.
Naı̈ve Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
naı̈ve cd4 t cell isolation kit - by Bioz Stars, 2026-02
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Inhibition of NLRP3 shifted the differentiation of naïve <t>CD4</t> + T cells toward a Treg phenotype in vitro. ( a ) Flow cytometry plots identifying Th17 and Treg populations in EAM versus MCC950-treated groups; ( b ) Bar graphs quantifying Th17 and Treg frequencies in EAM versus MCC950-treated groups; ( c ) Secreted IL-17 and IL-10 protein levels in culture supernatants; ( d ) RORγt and Foxp3 mRNA expression levels. Data are presented as mean ± SD, n = 3. NS indicates no statistical significance; ∗p < 0.05, ∗∗p < 0.01.
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Inhibition of NLRP3 shifted the differentiation of naïve <t>CD4</t> + T cells toward a Treg phenotype in vitro. ( a ) Flow cytometry plots identifying Th17 and Treg populations in EAM versus MCC950-treated groups; ( b ) Bar graphs quantifying Th17 and Treg frequencies in EAM versus MCC950-treated groups; ( c ) Secreted IL-17 and IL-10 protein levels in culture supernatants; ( d ) RORγt and Foxp3 mRNA expression levels. Data are presented as mean ± SD, n = 3. NS indicates no statistical significance; ∗p < 0.05, ∗∗p < 0.01.
Ca Cat, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Inhibition of NLRP3 shifted the differentiation of naïve <t>CD4</t> + T cells toward a Treg phenotype in vitro. ( a ) Flow cytometry plots identifying Th17 and Treg populations in EAM versus MCC950-treated groups; ( b ) Bar graphs quantifying Th17 and Treg frequencies in EAM versus MCC950-treated groups; ( c ) Secreted IL-17 and IL-10 protein levels in culture supernatants; ( d ) RORγt and Foxp3 mRNA expression levels. Data are presented as mean ± SD, n = 3. NS indicates no statistical significance; ∗p < 0.05, ∗∗p < 0.01.
Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
mouse - by Bioz Stars, 2026-02
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Inhibition of NLRP3 shifted the differentiation of naïve CD4 + T cells toward a Treg phenotype in vitro. ( a ) Flow cytometry plots identifying Th17 and Treg populations in EAM versus MCC950-treated groups; ( b ) Bar graphs quantifying Th17 and Treg frequencies in EAM versus MCC950-treated groups; ( c ) Secreted IL-17 and IL-10 protein levels in culture supernatants; ( d ) RORγt and Foxp3 mRNA expression levels. Data are presented as mean ± SD, n = 3. NS indicates no statistical significance; ∗p < 0.05, ∗∗p < 0.01.

Journal: Biochemistry and Biophysics Reports

Article Title: NLRP3 inflammasome regulates Th17/Treg cell balance in experimental autoimmune myocarditis

doi: 10.1016/j.bbrep.2026.102447

Figure Lengend Snippet: Inhibition of NLRP3 shifted the differentiation of naïve CD4 + T cells toward a Treg phenotype in vitro. ( a ) Flow cytometry plots identifying Th17 and Treg populations in EAM versus MCC950-treated groups; ( b ) Bar graphs quantifying Th17 and Treg frequencies in EAM versus MCC950-treated groups; ( c ) Secreted IL-17 and IL-10 protein levels in culture supernatants; ( d ) RORγt and Foxp3 mRNA expression levels. Data are presented as mean ± SD, n = 3. NS indicates no statistical significance; ∗p < 0.05, ∗∗p < 0.01.

Article Snippet: Naive CD4 + T cells were isolated using the specific naive CD4 + T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany)and cultured in 24-well plates at 1 × 10 6 cells/well in complete DMEM medium supplemented with 10 % FBS and 1 % penicillin-streptomycin.

Techniques: Inhibition, In Vitro, Flow Cytometry, Expressing